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anti itga7 alexa fluor 700  (R&D Systems)


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    Structured Review

    R&D Systems anti itga7 alexa fluor 700
    Anti Itga7 Alexa Fluor 700, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+itga7+alexa+fluor+700/Mouse+Integrin+alpha+7+Alexa+Fluor%C2%AE+700-conjugated+Antibody/bio_rxiv__64898__2026__04__21__719595-209-59-64
    Average 93 stars, based on 10 article reviews
    anti itga7 alexa fluor 700 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells.
    Article Snippet: .. 1990 Cell Reports 21, 1982–1993, November 14, 2017 tionally incubated 45 min on ice with conjugated anti-TER119-PECy7 (1.4 mg/mL; BD Biosciences, #557853), anti-CD45-PECy7 (1.4 mg/mL; BD Biosciences, #552848), anti-CD34-BV421 (5.6 mg/mL; BD Biosciences, #562608), anti-SCA-1-PE (3.5 mg/mL; BD Biosciences, #553108), and anti-ITGA7-Alexa Fluor 700 (0.56 mg/mL; R&D Systems, #FAB3518N) in DMEM (GIBCO)/0.2% BSA. ..

    Article Title: Postnatal Pax7-expressing limb cells are multipotent and generate non-myogenic lineages that persist into adulthood
    Article Snippet: For experiments performed at postnatal stages, muscle bulk filtration upon digestion was done only with 70 μm filters (Miltenyi Biotec, 130-110-916) into 15 ml tubes and the last centrifugation was performed at 600 g for 8 min at 4°C. .. Muscle bulks were blocked in 2% BSA in PBS for 15 min, centrifuged for 5 min/600 g at 4°C and were incubated on ice for 45 min protected from light, with the following conjugated antibodies diluted in 0.2% BSA in PBS: anti-TER119-PECy7 (3.5 μl, BD Biosciences, 557853) anti-CD45-PECy7 (3.5 μl, BD Biosciences, 552848), anti-Sca-1-PE (3.5 μl; BD Biosciences, 553108), anti-ITGA7-Alexa Fluor 700 (14 μl, R&D Systems, FAB3518N), anti-CD34-BV605 (14 μl, BD Biosciences, 750918) and anti-CD31-APC (2.66 μl, BD Biosciences, Cat # 551262) in a final volume of 200 μl. ..



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    R&D Systems anti itga7 alexa fluor 700
    Anti Itga7 Alexa Fluor 700, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+itga7+alexa+fluor+700/Mouse+Integrin+alpha+7+Alexa+Fluor%C2%AE+700-conjugated+Antibody/bio_rxiv__64898__2026__04__21__719595-209-59-64
    Average 93 stars, based on 1 article reviews
    anti itga7 alexa fluor 700 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems itga7
    Figure 1. Generation and characterization of StemRep mice (A and B) Genetic constructs of (A) Pax7-ZsGreen1-NLS and (B) Myf5-E2Crimson-NLS. Representation of the wild-type allele (top), targeted allele (middle), and knockin allele after flippase-FRT recombination (bottom). (C) Representative flow cytometry plots of MuSCs (red) derived from StemRep mice and the associated CD11b-/CD31-/CD45-/LY-6A/E/ <t>CD34+/ITGA7+</t> antigen signature. (D and E) Post-sort representative images of cytospin preparations (D) and quantification (E) of MuSCs isolated based on nuclear ZsGreen1 fluorescence that stained positive using antibodies for PAX7. ZsGreen1 reporter, green; PAX7, red; Nuclei (Hoechst), blue. Scale bar, 50 mm. n = 4 mice. Data presented are means ± SEM. ns = not significant in a two-tailed unpaired Student’s t test (E).
    Itga7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+itga7+alexa+fluor+700/Mouse+Integrin+alpha+7+Alexa+Fluor%C2%AE+700-conjugated+Antibody/pm38876109-216-42-43
    Average 93 stars, based on 1 article reviews
    itga7 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Figure 1. Generation and characterization of StemRep mice (A and B) Genetic constructs of (A) Pax7-ZsGreen1-NLS and (B) Myf5-E2Crimson-NLS. Representation of the wild-type allele (top), targeted allele (middle), and knockin allele after flippase-FRT recombination (bottom). (C) Representative flow cytometry plots of MuSCs (red) derived from StemRep mice and the associated CD11b-/CD31-/CD45-/LY-6A/E/ CD34+/ITGA7+ antigen signature. (D and E) Post-sort representative images of cytospin preparations (D) and quantification (E) of MuSCs isolated based on nuclear ZsGreen1 fluorescence that stained positive using antibodies for PAX7. ZsGreen1 reporter, green; PAX7, red; Nuclei (Hoechst), blue. Scale bar, 50 mm. n = 4 mice. Data presented are means ± SEM. ns = not significant in a two-tailed unpaired Student’s t test (E).

    Journal: Stem cell reports

    Article Title: A dual-color PAX7 and MYF5 in vivo reporter to investigate muscle stem cell heterogeneity in regeneration and aging.

    doi: 10.1016/j.stemcr.2024.05.005

    Figure Lengend Snippet: Figure 1. Generation and characterization of StemRep mice (A and B) Genetic constructs of (A) Pax7-ZsGreen1-NLS and (B) Myf5-E2Crimson-NLS. Representation of the wild-type allele (top), targeted allele (middle), and knockin allele after flippase-FRT recombination (bottom). (C) Representative flow cytometry plots of MuSCs (red) derived from StemRep mice and the associated CD11b-/CD31-/CD45-/LY-6A/E/ CD34+/ITGA7+ antigen signature. (D and E) Post-sort representative images of cytospin preparations (D) and quantification (E) of MuSCs isolated based on nuclear ZsGreen1 fluorescence that stained positive using antibodies for PAX7. ZsGreen1 reporter, green; PAX7, red; Nuclei (Hoechst), blue. Scale bar, 50 mm. n = 4 mice. Data presented are means ± SEM. ns = not significant in a two-tailed unpaired Student’s t test (E).

    Article Snippet: For initial validation of the PAX7/MYF5 model, cells were incubated at 4 C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen, #RM5228, 1/25), CD11b (Invitrogen, #RM2828, 1/25), CD34 (BD Biosciences, #560238, 1/60), Ly-6A/E (BD Biosciences, #561021, 1/150), and ITGA7 (R&D Systems, #FAB3518N, 1/30).

    Techniques: Construct, Knock-In, Cytometry, Derivative Assay, Isolation, Staining, Two Tailed Test